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倪其焜

研究員

倪其焜
Email: ckni[at]po.iams.sinica.edu.tw
Office: R406
Tel: +886-2-2366-8277
Lab: 320
Lab Tel: +886-2-2366-8268
Carbohydrate Mass Spectrometry Laboratory

研究興趣

1. 發展新型質譜技術研究醣分子結構
2. 基質輔助雷射脫附游離機制
3. 分子束中的有機分子、小型生化分子的光化學
4. 交叉分子束中高能量分子的能量轉移及超級碰撞

研究概述

倪其焜博士為質譜技術專家，其研究團隊認知到構成生命的巨分子之一 : 碳水化合物，至近期仍沒有一個快速有效的初級結構的鑑定方式，因此從2017 年起全力投入這個重要課題的研究。構成生命的三類巨分子 : 核糖核酸，蛋白質，及碳水化合物，至今只有碳水化合物依然沒有可靠快速的方法可以決定其初級結構。碳水化合物初級結構的鑑 定，傳統上是使用各種化學反應法和酵素，或是使用核磁共振光譜，但這兩種方法都耗時、耗樣品。 質譜儀有很高的靈敏度，所需的樣品非常少，但是傳統質譜法只能決定碳水化合物醣酐鍵的位置，無法決定變旋異構物、單醣的立體結構等。 倪博士實驗室研究團隊，結合實驗，量子化學計算，和化學動力學理論，研究碳水化合物在氣相的分解機制，並進一步利用這些分解機制，提出一個全新的質譜法，名為「邏輯演繹序列串聯質譜法」，可以鑑定醣酐鍵的位置、變旋異構物、單醣的立體結構等。 倪博士實驗室開發的方法，是目前全世界所有質譜法中，唯一可以鑑定寡糖完全初級結構的質譜方法。最近倪博士團隊將這個方法應用在各種生物體中，發現了前所未見的新寡糖。

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學歷

  • 民國74年，清華大學化學系學士
  • 民國82年，美國哥倫比亞大學化學系物理化學博士

經歷

  • 民國76至77年，臺灣清華大學化學系助教
  • 民國82至84年，美國加州大學柏克萊分校化學系博士後研究員
  • 民國87至91年，中央研究院原子與分子科學研究所助研究員
  • 民國91至95年，中央研究院原子與分子科學研究所副研究員
  • 民國95年迄今，中央研究院原子與分子科學研究所研究員
  • 民國93年至97年，臺灣清華大學化學系合聘副教授
  • 民國97年迄今，臺灣清華大學化學系合聘教授

獎項與榮譽

  • Hammett Awards & Hammett Fellowship, Chemistry Department, Columbia University, NY, NY U.S.A 1993
  • 民國96年，清華化學科技文教基金會傑出青年學者獎
  • 民國99年，國科會傑出研究獎
  • 民國108年，台灣質譜學會優秀學者研究獎
  • 民國110年，傑出人才基金會 傑出人才講座
代表著作

Structural Determination of Carbohydrates by Mass Spectrometry

  1. Chia Yen Liew, Hong-Sheng Luo, Ting-Yi Yang, An-Ti Hung, Bryan John Abel Magoling, Charles Pin-Kuang Lai, Chi-Kung Ni*, Identification of the high mannose N-glycan isomers undescribed by conventional multicellular eukaryotic biosyntheticpathways. Anal. Chem. 95, 23, 8789–8797 (2023)
  2. Shang-Ting Tsai, Hsu-Chen Hsu, and Chi-Kung Ni*. A simple tandem mass spectrometry method for structural identification of pentose oligosaccharides. Analyst, 148, 1712 (2023).
  3. Hock-Seng Nguan and Chi-Kung Ni*. Collision-induced dissociation of α-isomaltose and α-maltose. J. Phys. Chem. A 126, 8799−8808 (2022). (virtual special issue “Paul L. Houston Festschrift”)
  4. Chia Yen Liew, Jien-Lian Chen, Chi-Kung Ni*. Identification of side-reaction products generated during the ammonia-catalyzed release of N-glycans. Carbohydr. Res. 552, 108686, (2022).
  5. Chia Yen Liew, Hsu Chen Hsu, Hock-Seng Nguan, Yu-Chao Huang, Yong-Qing Zhong, Shang-Cheng Hung, and Chi-Kung Ni*. The good, the bad, and the ugly memories of carbohydrate fragments in collision-induced dissociation. J. Am. Soc. Mass Spectrom. 33, 1891-1903 (2022).
  6. Hai Thi Huynh, Shang-Ting Tsai, Po-Jen Hsu, Anik Biswas, Huu Trong Phan, Jer-Lai Kuo, Chi-Kung Ni*, and Cheng-chau Chiu*. Collision-induced dissociation of Na+-tagged ketohexoses: experimental and computational studies on fructose. Phys. Chem. Chem. Phys. 24, 20856-20866 (2022).
  7. Chia Yen Liew, Jien-Lian Chen, Chi-Kung Ni*. Electrospray ionization in‐source decay of N‐glycans and the effects on N‐glycan structural identification. Rapid Commun. Mass Spectrom. 36(18), e9352 (2022).
  8. Wei‑Chien Weng, Hung‑En Liao, Shih‑Pei Huang, Shang‑Ting Tsai, Hsu‑Chen Hsu, Chia Yen Liew, Veeranjaneyulu Gannedi, Shang‑Cheng Hung, Chi‑Kung Ni*, Unusual free oligosaccharides in human bovine and caprine milk. Scientific Reports, 12, 10790 (2022).|
  9. Hock-Seng Nguan, Shang-Ting Tsai, and Chi-Kung Ni*, Collision-Induced Dissociation of Cellobiose and Maltose. J. Phys. Chem. A 126, 1486-1495 (2022).
  10. Shang-Ting Tsai and Chi-Kung Ni*.Differentiation of aldohexoses and ketohexoses through collision‐induced dissociation. J. Chin. Chem. Soc. 69 (1), 173-183 (2022). (special issue for Prof. Yuan Pern Lee’s 70th Birthday)
  11. Hou-Yu Lin and Chi-Kung Ni*. Structural determination of polysaccharides lichenin using logically derived sequence tandem mass spectrometry. J. Am. Soc. Mass Spectrom. 33, 335−346 (2022).
  12. Shang-Ting Tsai, Hock-Seng Nguan, and Chi-Kung Ni*. Identification of anomericity and linkage of arabinose and ribose through collision-induced dissociation. J. Phys. Chem. A 125, 28, 6109-6121 (2021). (special issue of125 Years of The Journal of Physical Chemistry).
  13. Chi-Kung Ni*, Hsu Chen Hsu, Chia Yen Liew, Shih-Pei Huang, Shang-TingTsai. “Modern mass spectrometry techniques for oligosaccharide structure determination: Logically derived sequence tandem mass spectrometry for automatic oligosaccharide structural determination,” in Comprehensive Glycoscience, 2nd edition, edited by Joe Barchi, Elsevier, Oxford (2021)..
  14. Chia Yen Liew, Chu-Chun Yen, Jien-Lian Chen, Shang-Ting Tsai, Sujeet Pawar, Chung-Yi Wu*, Chi-Kung Ni*. Structural identification of N-glycan isomers using logically derived sequence tandem mass spectrometry. Commun. Chem. 4, 92 (2021).
  15. Shih-Pei Huang, Hsu Chen Hsu, Chia Yen Liew, Shang-Ting Tsai, Chi-Kung Ni*. Logically derived sequence tandem mass spectrometry for structural determination of galactose oligosaccharides. Glycoconj. J. 38, 177-189 (2021).
  16. Hock-Seng Nguan, Shang-Ting Tsai, Jien-Lian Chen, Po-Jen Hsu, Jer-Lai Kuo, and Chi-Kung Ni*. Collision-induced dissociation of xylose and its applications in linkage and anomericity identification. Phys. Chem. Chem. Phys. 23, 3485 (2021).
  17. Chi-Kung Ni*, Hsu Chen Hsu, Chia Yen Liew, Shih-Pei Huang, Shang-Ting Tsai. Modern Mass Spectrometry Techniques for Oligosaccharide Structure Determination: Logically derived sequence tandem mass spectrometry for automatic oligosaccharide structural determination in Comprehensive Glycoscience, 2nd edition, edited by Joseph J. Barchi, Jr., Elsevier, Oxford, (2021).
  18. Cheng-chau Chiu, Hai Thi Huynh, Shang-Ting Tsai, Hou-Yu Lin, Po-Jen Hsu, Huu Trong Phan, Arya Karumanthra, Hayden Thompson, Yu-Chi Lee, Jer-Lai Kuo*, Chi-Kung Ni*. Towards closing the gap between hexoses and N-acetlyhexosamines: experimental and computational studies on the collision-induced dissociation of hexosamines. J. Phys. Chem. A 123, 6683-6700 (2019).
  19. Shang-Ting Tsai, Chia Yen Liew, Hsu Chen Hsu, Shih-Pei Huang, Wei-Chien Weng, Yu-Hsiang Kuo, and Chi-Kung Ni*. Automatic Full Glycan Structural Determination through Logically Derived Sequence Tandem Mass Spectrometry. ChemBioChem. 20, 2351-2359 (2019).
  20. Hsu Chen Hsu, Shih-Pei Huang, Chia Yen Liew, Shang-Ting Tsai, and Chi-Kung Ni*, De novo structural determination of mannose oligosaccharides by using a logically derived sequence for tandem mass spectrometry. Anal. Bioanal. Chem. 411, 3241 (2019).
  21. Cheng-chau Chiu, Shang-Ting Tsai, Po-Jen Hsu, Hai Thi Huynh, Jien-Lian Chen, Huu Trong Phan, Shih-Pei Huang, Hou-Yu Lin, Jer-Lai Kuo*, and Chi-Kung Ni*, Unexpected dissociation mechanism of sodiated N-acetylglucosamine and N-acetylgalactosamine. J. Phys. Chem. A 123, 3441 (2019).
  22. Yu-Hsuan Shen, Shang-Ting Tsai, Chia Yen Liew, Chi-Kung Ni*, Mass spectrometry-based identification of carbohydrate anomeric configuration to determine the mechanism of glycoside hydrolases. Carbohydr. Res. 476, 53-59 (2019).
  23. Hai Thi Huynh, Huu Trong Phan, Po-Jen Hsu, Jien-Lian Chen, Hock Seng Nguan, Shang-Ting Tsai, Thantip Roongcharoen, Chia Yen Liew, Chi-Kung Ni* and Jer-Lai Kuo*. Collision-induced dissociation of sodiated glucose, galactose, and mannose, and the identification of anomeric configurations. Phys. Chem. Chem. Phys. 20, 19614 (2018).
  24. Hsu Chen Hsu, Chia Yen Liew, Shih-Pei Huang, Shang-Ting Tsai, Chi-Kung Ni*. Simple Method for De Novo Structural Determination of Underivatised Glucose Oligosaccharides. Sci. Rep. 8, 5562 (2018).
  25. Hsu Chen Hsu, Chia Yen Liew, Shih-Pei Huang, Shang-Ting Tsai, Chi-Kung Ni*. Simple Approach for De Novo Structural Identification of Mannose Trisaccharides. J. Am. Soc. Mass. Spectrom. 29, 470 (2018).
  26. 1Jien-Lian Chen, Hock Seng Nguan, Po-Jen Hsu, Shang-Ting Tsai, Chia Yen Liew, Jer-Lai Kuo, Wei-Ping Hu, and Chi-Kung Ni*. Collision-induced dissociation of sodiated glucose and identification of anomeric configuration. Phys. Chem. Chem. Phys. 19, 15454 (2017).
  27. Shang-Ting Tsai, Jien-Lian Chen, and Chi-Kung Ni*. Does low-energy collision-induced dissociation of lithiated and sodiated carbohydrates always occur at anomeric carbon of reducing end? Rap. Commun. Mass Spectrom. 31, 1835–1844.(2017).

 
MALDI Ionization Mechanisms

  1. Hou-Yu Lin, Yuri A. Dyakov, Yuan Tseh Lee, Chi-Kung Ni*, Temperature dependence of desorbed ions and neutrals and ionization mechanism of matrix-assisted laser desorption/ionization. J. Am. Soc. Mass. Spectrom. (Accepted)
  2. Chuping Lee, Chi-Kung Ni*, Soft matrix–assisted laser desorption/ionization for labile glycoconjugates. J. Am. Soc. Mass. Spectrom. 30,1455-1463 (2019).
  3. Sheng-Ping Liang, I-Chung Lu, Shang-Ting Tsai, Jien-Lian Chen, Yuan Tseh Lee, Chi-Kung Ni*. Laser Pulse Width Dependence and Ionization Mechanism of Matrix-Assisted Laser Desorption/Ionization. J. Am. Soc. Mass. Spectrom. 28, 2235-2245 (2017).
  4. C. Lee, I. C. Lu, H. C. Hsu, H. Y. Lin, S. P. Liang, Y. T. Lee, C. K. Ni*. Formation of Metal-Related Ions in Matrix-Assisted Laser Desorption Ionization. J. Am. Soc. Mass Spectrom. 27, 1491-1498 (2016).
  5. I-Chung Lu, Chuping Lee, Yuan-Tseh Lee, and Chi-Kung Ni*, Ionization Mechanism of Matrix-Assisted Laser Desorption/Ionization. Ann. Rev. Anal. Chem. 8, 21-39 (2015).
  6. I-Chung Lu, Kuan Yu Chu, Chih-Yuan Lin, Shang-Yun, Wu, Yuri A. Dyakov, Jien-Lian Chen, Angus Gray-Weale, Yuan-Tseh Lee, Chi-Kung Ni*, Ion-to-Neutral Ratios and Thermal Proton Transfer in Matrix-Assisted Laser Desorption/Ionization. J. Am. Soc. Mass Spectrom. 26, 1242-1251 (2015).
  7. I-Chung Lu, Chuping Lee, Hui-Yuan Chen, Hou-Yu Lin, Sheng-Wei Hung, Yuri A. Dyakov, Kuo-Tung Hsu, Chih-Yu Liao, Yin-Yu Lee, Chien-Ming Tseng, Yuan-Tseh Lee, and Chi-Kung Ni*, Ion Intensity and Thermal Proton Transfer in Ultraviolet Matrix-Assisted Laser Desorption/Ionization. J. Phys. Chem. B 118, 4132 (2014).
  8. Hou-Yu Lin, Hsu Chen Hsu, I-Chung Lu, Kuo-Tung Hsu, Chih-Yu Liao Yin-Yu Lee, Chien-Ming Tseng, Yuan-Tseh Lee, and Chi-Kung Ni*, Fluorescence spectroscopy of UV-MALDI matrices and implications of ionization mechanisms, J. Chem. Phys. 141, 164307 (2014).
  9. Kuan Yu Chu, Sheng Lee, Ming-Tsang Tsai, I-Chung Lu, Yuri A. Dyakov, Yin Hung Lai, Yuan-Tseh Lee, Chi-Kung Ni*, Thermal Proton Transfer Reactions in Ultraviolet Matrix-Assisted Laser Desorption/Ionization. J. Am. Soc. Mass Spectrom. 25, 310 (2014).
  10. Hou-Yu Lin, Botao Song, I-Chung Lu1 Kuo-Tung Hsu, Chih-Yu Liao, Yin-Yu Lee, Chien-Ming Tseng, Yuan-Tseh Lee, Chi-Kung Ni*, Is energy pooling necessary in ultraviolet matrix-assisted laser desorption/ionization? Rapid Commun. Mass Spectrom. 28, 77 (2014).
  11. Ming-Tsang Tsai, Sheng Lee, I-Chung Lu, Kuan Yu Chu1, Chi-Wei Liang, Chih Hao Lee, Yuan T. Lee, and Chi-Kung Ni*, Ion-to-neutral ratio of 2,5-dihydroxybenzoic acid in matrix-assisted laser desorption/ionization. Rapid Commun. Mass Spectrom. 27, 955 (2013).

 
Energy Transfer of Highly Excited Molecules in Molecular Beams
 

  1. Chih-Yuan Lin, Hou-Yu Lin, Chi-Kung Ni*. Signal decay and recovery in matrix-assisted laser desorption/ionization and the thermal model. Int. J. Mass Spectrom. 486, 117011 (2023). (invited article for special issue to honor the 20th anniversary of the Nobel prize for ESI and MALDI.)
  2. Hou-Yu Lin, Yuri A. Dyakov, Yuan Tseh Lee, Chi-Kung Ni*. Temperature dependence of desorbed ions and neutrals and ionization mechanism of matrix-assisted laser desorption/ionization. J. Am. Soc. Mass. Spectrom. 32, 95-105 (2021).
  3. Hou-Yu Lin, Botao Farren Song, Chi-Kung Ni*. Fluorescence quantum yields of matrices used in ultravioletmatrix-assisted laser desorption/ionization. Rapid Commun Mass Spectrom.34, e8846 (2020).
  4. Hsu, Ming-Tsang Tsai, Yuri A. Dyakov, and Chi-Kung Ni*, Energy transfer of highly vibrationally excited molecules studied by crossed molecular beam/time-sliced velocity map ion imaging, Int. Rev. Phys. Chem. 31, 201, (2012).
  5. Hsu Chen Hsu, Jia-Jia Lyu, Chen-Lin Liu, Cheng-Liang Huang, and Chi-Kung Ni*, Generation and characterization of highly vibrationally excited molecular beam. J. Chem. Phys. 124, 054301 (2006).
  6. Chen-Lin Liu, Hsu Chen Hsu, Jia-Jia Lyu, and Chi-Kung Ni*, Energy transfer of highly vibrationally excited azulene: collisions between azulene and krypton. J. Chem. Phys. 124, 054302 (2006).
  7.  Chen-Lin Liu, Hsu Chen Hsu, Jia-Jia Lyu, and Chi-Kung Ni*, Supercollisions and energy transfer of highly vibrationally excited molecules. J. Chem. Phys. 123, 131102 (2005).

 
Photodissociation of Organic Molecules in Molecular Beam

  1. Yen-Cheng Lin, Chin Lee, Shih-Huang Lee, Yin-Yu Lee, Yuan T. Lee, Chien-Ming Tseng*, and Chi-Kung Ni*. Excited-state dissociation dynamics of phenol studied by a new time-resolved technique. J. Chem. Phys. 148, 074306 (2018).
  2. Chin Lee, Yen-Cheng Lin, Shih-Huang Lee, Yin-Yu Lee, Chien-Ming Tseng, Yuan-Tseh Lee, and Chi-Kung Ni*. Advantage of spatial map ion imaging in the study of large molecule photodissociation. J. Chem. Phys. 147, 013904 (2017).
  3. Yi Lin Yang, Yu-Chieh Ho, Yuri A. Dyakov, Wen-Hsin Hsu, Chi-Kung Ni*, Yi-Lun Sun, Wan-Chen Tsai and Wei-Ping Hu*, Effects of intramolecular hydrogen bonding on the excited state dynamics of phenol chromophores. Phys. Chem. Chem. Phys. 15, 7182 (2013).
  4. Chi-Kung Ni*, Cheng-Ming Tseng, Ming-Fu Lin, and Yuri Dyakov, Photodissociation dynamics of small aromatic molecules studied by multimass ion imaging. J. Phys. Chem. B 111, 12631 (2007) (invited Feature Article)
  5. Chien-Ming Tseng, Yuri A. Dyakov, Cheng-Liang Huang, Alexander M. Mebel, Sheng Hsien Lin, Yuan T. Lee, and Chi-Kung Ni*, Photoisomerization and photodissociation of aniline and 4-methylpyridine. J. Am. Chem. Soc. 126, 8760 (2004).
  6. Cheng-Liang Huang, Jyh-Chiang Jiang, Alex Mebel, Yuan T. Lee, and Chi-Kung Ni*, Photodissociation dynamics of fluorobenzene. J. Am. Chem. Soc. 125, 9814, (2003).
  7. Chi-Kung Ni* and Yuan T. Lee, Photodissociation of simple aromatic molecules in a Molecular Beam, Int. Rev. Phys. Chem. 23, 187 (2004).
  8. Shang-Ting Tsai, Chih-Kai Lin, Yuan T. Lee and Chi-Kung Ni*, Multimass Ion Imaging Detection: Application to Photodissociation. Rev. Sci. Instrum. 72, 1963, (2001).